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a horseradish peroxidase conjugated mouse anti-Human IL-6 Antibody is added to the wells and binds to IL-6 in the sample
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 54 nm
The Mouse EGFR (Epidermal Growth Factor Receptor) ELISA kit uses the Sandwich-ELISA principle
The OD value is proportional to the concentration of Mouse GROalpha/CXCL1
You can calculate the concentration of Human CASP1 in the samples by comparing the OD of the samples to the standard curve
Rat ADORA2a (Adenosine A2a Receptor) ELISA Kit (Small Sample) PC-I a horseradish peroxidase conjugated mouseThe Rat ADORA2a (Adenosine A2a Receptor) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat ADORA2a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat ADORA2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
