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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIF
The plate has been pre-coated with Human WIF1 antibody
only those wells that contain Rat EGFR
The plate has been pre-coated with Human TLR1 antibody
Substrate solution is then added and color develops in proportion to the amount of Human IRF1
Bovine IL-1b (Interleukin 1 Beta) ELISA Kit Serine Proteinase Inhibitor Standards or samples are addedThe Bovine IL 1b (Interleukin 1 Beta) ELISA Kit measures IL 1Beta in Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 1b antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
