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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL3
Substrate solution is then added and color develops in proportion to the amount of Rat PPARGC1A
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1qL3
The plate has been pre-coated with Human CYLC1 antibody
The concentration of Dog PDGFBB in the samples is then determined by comparing the OD of the samples to the standard curve
Rat PLIN1 (Perilipin-1) ELISA Kit PYCD The micro ELISA plate providedThe Rat (PLIN1) Perilipin 1 ELISA Kit measures Perilipin 1 in Rat samples. The plate has been pre coated with Rat PLIN1 antibody. PLIN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PLIN1 Antibody is added and binds to PLIN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PLIN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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