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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LRP5
One aliquot is incubated at 0C (ice bath) and the other is incubated at 37C
Avi tag at the C-terminus
A standard curve is constructed by plotting absorbance values against procalcitonin concentrations of standards and concentrations of unknown samples are determined using this standard curve
Recombinant Human Folate Receptor Alpha is produced by our Mammalian expression system and the target gene encoding Arg25-Ser234 is expressed with a 6His tag at the C-terminus
Bovine PROG (Progesterone) ELISA Kit IL-1RT-2 Standards or samples are addedThe Bovine (PROG) Progesterone ELISA Kit measures Progesterone in Bovine samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and
