Human CST5 (Cystatin-D) ELISA Kit Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Mouse RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The plate has been pre-coated with Human CD151 antibody

The concentration of Human HABP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNF-alpha

Then Streptavidin-HRP is added and binds to the Biotinylated AKT1 antibody

Human CST5 (Cystatin-D) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (CST5) Cystatin D ELISA Kit measures Cystatin D in samples. The plate has been pre coated with Human CST5 antibody. CST5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CST5 Antibody is added and binds to CST5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CST5 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.