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FGF23 present in the sample is added and binds to antibodies coated on the wells
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG3 are added to the micro ELISA plate wells
The OD value is proportional to the concentration of Human CHEM
The OD value is proportional to the concentration of Mouse NOS2/iNOS
The concentration of Human RESP18 in the samples is then determined by comparing the OD of the samples to the standard curve
Human WNT5B (Protein Wnt-5B) ELISA Kit Packaging:96 wells FGF23 present in the sampleThe Human (WNT5B) Protein Wnt 5B ELISA Kit measures Protein Wnt 5B in samples. The plate has been pre coated with Human WNT5B antibody. WNT5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human WNT5B Antibody is added and binds to WNT5B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated WNT5B antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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