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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human TFR1 are added to the micro ELISA plate wells
The OD value is proportional to the concentration of sST2
Only those wells that contain Human MMP-7
The concentration of Rat GPA33 in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Human TICAM1
Bovine NASP (Nuclear Autoantigenic Sperm Protein) ELISA Kit Packaging:96 wells Samples (or Standards) and HorseradishThe Bovine (NASP) Nuclear Autoantigenic Sperm Protein ELISA Kit measures Nuclear Autoantigenic Sperm Protein in Bovine samples. The plate has been pre coated with Bovine NASP antibody. NASP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine NASP Antibody is added and binds to NASP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NASP antibody. After incubation unbound
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