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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep IL12
The micro ELISA plate provided in this kit has been pre-coated with Universal VA
Then a biotinylated detection antibody specific for Mouse OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Human SYNPO
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CTGF
Rat LH (Luteinizing Hormone) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Rat (LH) Luteinizing Hormone ELISA Kit measures Luteinizing Hormone in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added
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