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Substrate solution is then added and color develops in proportion to the amount of Human AHNAK2
Then TMB substrate solution is added to each well
Calculate the concentration of VDR in the sample by plotting standard curve
This IL-3 (Interleukin-3) ELISA Kit (Mini-Sample) uses the sandwich-ELISA principle
And then biotinylated Human AKR1B1 Antibody is added and binds to AKR1B1 in the sample
Porcine IL-22 (Interleukin 22) ELISA Kit Small-inducible cytokine B5 Substrate solution is then addedThe Porcine IL 22 (Interleukin 22) ELISA Kit measures IL 22 in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 22 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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