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the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue colored product that is directly proportional to the amount of IGFBP-1 present
The concentration of Human MIF in the samples is then determined by comparing the OD of the samples to the standard curve
The Human IL-5 ELISA kit is a sandwich enzyme immunoassay
The concentration of Human CD163 in the samples is then determined by comparing the OD of the samples to the standard curve
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PROC
GZMH (Granzyme H) ELISA Kit (Small Sample) IL-1R-alpha the TMB substrate (enzyme substrate)The GZMH (Granzyme H) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human GZMH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human GZMH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only
