Human IFN-Gamma (Interferon gamma) QuickTest ELISA Kit nuclear receptor subfamily 1 The antigens in the samples

The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Fcγ

The concentration of Cattle IFNg in the samples is then determined by comparing the OD of the samples to the standard curve

Substrate solution is then added and color develops in proportion to the amount of Rat HSPD1

only those wells that contain Human MS4A4A

Human IFN-Gamma (Interferon gamma) QuickTest ELISA Kit nuclear receptor subfamily 1 The antigens in the samplesThe Human IFN Gamma (Interferon gamma) QuickTest ELISA Kit measures IFN Gamma in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples