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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDGF-BB
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GOLGA8A
The plate has been pre-coated with Human FBXO32 antibody
Then a biotinylated detection antibody specific for Human THP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of CNTF in the samples is then determined by comparing the OD of the samples to the standard curve
Human KLK12 (Kallikrein-12) ELISA Kit Packaging:96 wells The micro ELISA plate providedThe Human (KLK12) Kallikrein 12 ELISA Kit measures Kallikrein 12 in samples. The plate has been pre coated with Human KLK12 antibody. KLK12 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KLK12 Antibody is added and binds to KLK12 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KLK12 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
