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Substrate solution is then added and color develops in proportion to the amount of Human KCNAB2
The concentration of Universal DA in tested samples can be calculated by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL13
CILP present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Human VWFCP/ADAMTS13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human KRT23 (Keratin 23) ELISA Kit LOXIN Substrate solution is then addedThe Human (KRT23) Keratin 23 ELISA Kit measures Keratin 23 in samples. The plate has been pre coated with Human KRT23 antibody. KRT23 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KRT23 Antibody is added and binds to KRT23 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KRT23 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
