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Mouse BNP in the sample or standard competes with a fixed amount of Mouse BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse BNP
only those wells that contain Human MIP5
The OD value is proportional to the concentration of Human CX3CL1
Then a biotinylated detection antibody specific for Human VCAN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Rat IFN-gamma
Goat IFN-alpha ELISA Kit Packaging:48 wells Mouse BNP in the sampleThe Goat IFN alpha ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Goat IFN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Goat IFN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those
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