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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 33 nm
The Bovine (NANOG) Homeobox Protein Nanog ELISA Kit measures Homeobox Protein Nanog in Bovine samples
The concentration of Human EXOSC7 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse TGFb3
which usually recognizes the much larger protein domain
Mouse NPHS2 (Podocin) ELISA Kit Packaging:96 wells The optical density (OD) isThe Mouse (NPHS2) Podocin ELISA Kit measures Podocin in Mouse samples. The plate has been pre coated with Mouse NPHS2 antibody. NPHS2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NPHS2 Antibody is added and binds to NPHS2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NPHS2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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