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Then a biotinylated detection antibody specific for Human CD30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Substrate solution is then added and color develops in proportion to the amount of Human BIN3
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65
You can calculate the concentration of Rat CNTF in the samples by comparing the OD of the samples to the standard curve
NMI present in the sample is added and binds to antibodies coated on the wells
Mouse NRG2 (Neuregulin-2) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Mouse (NRG2) Neuregulin 2 ELISA Kit measures neuregulin 2 in Mouse samples. The plate has been pre coated with Mouse NRG2 antibody. NRG2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NRG2 Antibody is added and binds to NRG2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NRG2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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