Then a biotinylated detection antibody specific for Human DKK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of CX3CL1 in the samples is then determined by comparing the OD of the samples to the standard curve
a capture antibody-CCL25-biotin-detection antibody complex was formed
The plate has been pre-coated with Goat IL18 antibody
Calculate the concentration of IL36G in the sample by plotting standard curve
EDN1 (Endothelin-1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe EDN1 (Endothelin 1) ELISA Kit measures ET 1 in Mouse, Rat, Rabbit, Goat, Canine, Human, Porcine, Bovine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ET 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound