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NEUROD1 present in the sample is added and binds to antibodies coated on the wells
a horseradish peroxidase conjugated mouse anti-Mouse TNFR-2 Antibody is added to the wells and binds to TNFR-2 in the sample
The concentration of Rat ECF in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Rat Ntn1
The OD value is proportional to the concentration of Human CA125
Human PDCD1 (Programmed Cell Death Protein 1) ELISA Kit Packaging:96 wells NEUROD1 present in the sampleThe Human (PDCD1) Programmed Cell Death Protein 1 ELISA Kit measures Programmed Cell Death Protein 1 in samples. The plate has been pre coated with Human PDCD1 antibody. PDCD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PDCD1 Antibody is added and binds to PDCD1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PDCD1 antibody. After incubation unbound Streptavidin
