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Substrate solution is then added and color develops in proportion to the amount of Bovine PF-4
Then a biotinylated detection antibody specific for HO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIF
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-γ
The OD value is proportional to the concentration of Human MDC/CCL22
Human KLB (Klotho Beta) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (KLB) Klotho Beta ELISA Kit measures Klotho Beta in samples. The plate has been pre coated with Human KLB antibody. KLB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KLB Antibody is added and binds to KLB in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KLB antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
