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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL4
Then a biotinylated detection antibody specific for Mouse GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human MT in the sample or standard competes with a fixed amount of Human MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human MT
The concentration of Dog IL2 in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human CLDN3
Porcine Leptin ELISA Kit Packaging:96 wells The microtiter plate provided inThe Porcine Leptin ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Pig LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Pig LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells
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