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Then a biotinylated detection antibody specific for Human FDP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat DAO
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Pro-ANP
Then a biotinylated detection antibody specific for Rat SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMP8B
Human VCL (Vinculin) ELISA Kit Osteoblast Specific Factor 2 Then a biotinylated detection antibodyThe Human (VCL) Vinculin ELISA Kit measures vinculin in samples. The plate has been pre coated with Human VCL antibody. VCL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human VCL Antibody is added and binds to VCL in the sample. Then Streptavidin HRP is added and binds to the Biotinylated VCL antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
