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Then a biotinylated detection antibody specific for Rat VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The assay plate has been pre-coated with mouse anti-Human HAI-1 monoclonal antibody
and the absorbance is spectrophotometrically measured at 450nm
only those wells that contain Mouse APOB100
Only those wells that contain Mouse Tn-I
Rat ADIPOR2 (Adiponectin Receptor 2) ELISA Kit (Small Sample) Packaging:48 wells Then a biotinylated detection antibodyThe Rat ADIPOR2 (Adiponectin Receptor 2) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat ADIPOR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat ADIPOR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
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