Mouse VCAM-1 (Vascular cell adhesion molecule 1) ELISA Kit (Mini-Sample) GC-B Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CLCF1

coli expression system and the target gene encoding Ser19-Ser178 is expressed

The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450+/-2 nm

The concentration of Rat MTL in tested samples can be calculated by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Rat VEGFR-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Mouse VCAM-1 (Vascular cell adhesion molecule 1) ELISA Kit (Mini-Sample) GC-B Standards or samples are addedThis Mouse VCAM 1 (Vascular cell adhesion molecule 1) ELISA Kit (Mini Sample) uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to VCAM 1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VCAM 1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to