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Then a biotinylated detection antibody specific for Rat IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFa4
The concentration of Human SCGN in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Human EGF in the samples is then determined by comparing the OD of the samples to the standard curve
The microplate provided in this kit has been precoated with anti ABCB5 antibody
Human IA-2 Autoantibody ELISA Kit SK Then a biotinylated detection antibodyThe Human IA 2 Autoantibody ELISA Kit measures IA 2 Autoantibody in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP
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