Human ACTN1 (Alpha-actinin-1) ELISA Kit CTAP-III Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTOR

Then Streptavidin-HRP is added and binds to the Biotinylated KRT2 antibody

Smad1 present in the sample is added and binds to antibodies coated on the wells

And then biotinylated Human CHST7 Antibody is added and binds to CHST7 in the sample

Substrate solution is then added and color develops in proportion to the amount of Mouse LTB4R2

Human ACTN1 (Alpha-actinin-1) ELISA Kit CTAP-III Standards or samples are addedThe Human ACTN1 (Alpha actinin 1) ELISA Kit measures ACTN1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ACTN1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection