Human PDE4A (cAMP-specific 3',5'-cyclic phosphodiesterase 4A) ELISA Kit Lymphotoxin Alpha Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Rat AGER

only those wells that contain Rabbit FGF2

The OD value is proportional to the concentration of Mouse TNNI3/cTn-I

Then Streptavidin-HRP is added and binds to the Biotinylated CTF2 antibody

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FCN2

Human PDE4A (cAMP-specific 3',5'-cyclic phosphodiesterase 4A) ELISA Kit Lymphotoxin Alpha Substrate solution is then addedThe Human PDE4A (cAMP specific 3',5' cyclic phosphodiesterase 4A) ELISA Kit measures PDE4A in Human Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PDE4A antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.