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The OD value is proportional to the concentration of Mouse BMG/β2-MG
Substrate solution is then added and color develops in proportion to the amount of Mouse DPP4
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CFH
The plate has been pre-coated with Rat CA3 antibody
The GRAP2 (GRB2 Related Adaptor Protein 2) ELISA Kit is a Sandwich enzyme immunoassay
Rat IgM (Immunoglobulin M) ELISA Kit Packaging:96 wells The OD value is proportionalThe Rat (IgM) Immunoglobulin M ELISA Kit measures Immunoglobulin M in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and
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