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The concentration of Rat UCP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated SPX antibody
Then a biotinylated detection antibody specific for Human COL3α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL9a3
Porcine E-Selectin ELISA Kit SCYB9B The concentration of Rat UCP1The Porcine E Selectin ELISA Kit measures E Selectin in Porcine samples. The plate has been pre coated with Porcine SELE antibody. SELE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine SELE Antibody is added and binds to SELE in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SELE antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
