Human PCK1 (Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit SCYB2 GLO1 present in the sample

GLO1 present in the sample is added and binds to antibodies coated on the wells

Only those wells that contain Human TWEAK

it binds with PDP bound to precoated antibody

Then Streptavidin-HRP is added and binds to the Biotinylated LRP8 antibody

Calculate the concentration of FLG in the sample by plotting standard curve

Human PCK1 (Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit SCYB2 GLO1 present in the sampleThe Human PCK1 (Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit measures PCK1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PCK1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.