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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPO
And then biotinylated Human EAF2 Antibody is added and binds to EAF2 in the sample
And then biotinylated Human HMGN4 Antibody is added and binds to HMGN4 in the sample
only those wells that contain Human DEFa3
The concentration of Human FETUA in the samples is then determined by comparing the OD of the samples to the standard curve
Human SPON2 (Spondin-2) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Human (SPON2) Spondin 2 ELISA Kit measures Spondin 2 in samples. The plate has been pre coated with Human Spon2 antibody. Spon2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Spon2 Antibody is added and binds to Spon2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Spon2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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