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Then a biotinylated detection antibody specific for Human AGP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
GKN2 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated CD244 antibody
And then biotinylated Mouse SPRN Antibody is added and binds to SPRN in the sample
And then biotinylated Porcine IFNAR1 Antibody is added and binds to IFNAR1 in the sample
Human IMA (Ischemia Modified Albumin) QuickTest ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human IMA (Ischemia Modified Albumin) QuickTest ELISA Kit measures IMA in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
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