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The concentration of Rat LCT in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A11
After washing
And then biotinylated Human GABARAPL2 Antibody is added and binds to GABARAPL2 in the sample
Human GKN1 (Gastrokine 1) ELISA Kit Packaging:96 wells The concentration of Rat LCTThe Human (GKN1) Gastrokine 1 ELISA Kit measures Gastrokine 1 in samples. The plate has been pre coated with Human GKN1 antibody. GKN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GKN1 Antibody is added and binds to GKN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GKN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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