Mal-Propionyl-PEG2-Val-Ala-PAB(m-PEG4)-PNP mpeg 95 SMILES CC(C)(C)OC(=O)CN1CCN(CC(=O)NCCNC(=O)CCN2C(=O)C=CC2=O)CCN(CC(=O)OC(C)(C)C)CCN(CC(=O)OC(C)(C)C)CC1 Purity (HPLC)

95 SMILES CC(C)(C)OC(=O)CN1CCN(CC(=O)NCCNC(=O)CCN2C(=O)C=CC2=O)CCN(CC(=O)OC(C)(C)C)CCN(CC(=O)OC(C)(C)C)CC1 Purity (HPLC) >= 95% Shipping Conditions Room temperature Storage Conditions Refrigerated Shelf Life 12 months after the date of delivery Regulatory Statement For Research Use Only

93 SMILES ClCCOCCOCCOCCC(=O)NC1=C2CN(C3CCC(=O)NC3=O)C(=O)C2=CC=C1 Purity (HPLC) >= 95% Shipping Conditions Room temperature Storage Conditions Refrigerated Shelf Life 12 months after the date of delivery Regulatory Statement For Research Use Only

Product Name Propargyl-Gly(m-PEG4)-Gly-Gly-Phe-Gly-PAB(m-PEG4)-MMAE Synonyms CAS Number Related CAS Molecular Formula C87H134N12O24 Molecular Weight 1732

Excitation maximum = 558 nm

Methyltetrazine is typically used in the inverse electron-demand Diels-Alder (iEDDA) reaction

Mal-Propionyl-PEG2-Val-Ala-PAB(m-PEG4)-PNP mpeg 95 SMILES CC(C)(C)OC(=O)CN1CCN(CC(=O)NCCNC(=O)CCN2C(=O)C=CC2=O)CCN(CC(=O)OC(C)(C)C)CCN(CC(=O)OC(C)(C)C)CC1 Purity (HPLC)SOLU KNEX cleavable linker features a Val Ala dipeptide. The conventional maleimidocaproyl (MC) unit is replaced by Mal Propionyl PEG2 moiety to enhance aqueous solubility. The Mal Propionyl group may also improve retro Michael addition stability during antibody conjugation. m PEG4 is attached to the commonly used self immolative para aminobenzyl (PAB) moiety to increase aqueous solubility. The PNP (para nitrophenyl) moiety serves as an activating