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it binds with AAPA bound to precoated antigen
PDI present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Human CSPG4
Substrate solution is then added and color develops in proportion to the amount of Rat CACNA2D1
Calculate the concentration of Ache in the sample by plotting standard curve
Mouse CA2 (Carbonic Anhydrase 2) ELISA Kit Packaging:96 wells it binds with AAPA boundThe Mouse CA2 (Carbonic Anhydrase 2) ELISA Kit measures CA2 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CA2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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