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a capture antibody-APOA1-biotin-detection antibody complex was formed
catestatin present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Human CS
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOB
a capture antibody-VIP-biotin-detection antibody complex was formed
Guinea pig C3a (Complement Component 3a) ELISA Kit Packaging:96 wells a capture antibody-APOA1-biotin-detection antibody complexThe Guinea pig C3a (Complement Component 3a) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig C3a and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to
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