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PDG in the sample or standard competes with a fixed amount of biotin-labeled PDG for sites on a pre-coated antibody specific to PDG
Only those wells that contain Porcine TFPI2
a horseradish peroxidase conjugated mouse anti-Human ST2 Antibody is added to the wells and binds to ST2 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated ADAMTS14 antibody
You can calculate the concentration of Porcine GSTAlpha1 in the samples by comparing the OD of the samples to the standard curve
Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit Packaging:48 wells PDG in the sample orThe Human TIMP 1 (Tissue Inhibitors of Metalloproteinase 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human TIMP 1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP 1 and Avidin Horseradish Peroxidase (HRP) conjugate are added
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