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SHBG present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Human TRAF6 Antibody is added and binds to TRAF6 in the sample
Substrate solution is then added and color develops in proportion to the amount of Mouse THBS2
And then biotinylated Mouse IL21R Antibody is added and binds to IL21R in the sample
The OD value is proportional to the concentration of Mouse IL-23
Human UCP3 (Mitochondrial Uncoupling Protein 3) ELISA Kit Packaging:96 wells SHBG present in the sampleThe Human (UCP3) Mitochondrial Uncoupling Protein 3 ELISA Kit measures Mitochondrial Uncoupling Protein 3 in samples. The plate has been pre coated with Human UCP3 antibody. UCP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human UCP3 Antibody is added and binds to UCP3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated UCP3 antibody. After incubation unbound Streptavidin
