Human Tumor Necrosis Factor (TNF) ELISA Kit In-Fusion Cloning The OD value is proportional

The OD value is proportional to the concentration of Human RANTES

Only those wells that contain Human COL1α2

The OD value is proportional to the concentration of Rat IgG2c

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IFN Gamma

Then a biotinylated detection antibody specific for Mouse TNFRSF1B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human Tumor Necrosis Factor (TNF) ELISA Kit In-Fusion Cloning The OD value is proportionalThis ELISA kit is designed to detect Human Tumor Necrosis Factor (Human TNF). The assay plate has been pre coated with mouse anti Human TNF alpha monoclonal antibody. When the sample containing TNF alpha is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti Human TNF alpha Antibody is added to the wells and binds to TNF alpha in the sample. After washing the wells, substrate