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PNMT present in the sample is added and binds to antibodies coated on the wells
The OD value is proportional to the concentration of NGAL
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CPA2
The Human (ITIH4) Inter-alpha-trypsin Inhibitor Heavy Chain H4 ELISA Kit measures Inter-alpha-trypsin inhibitor heavy chain H4 in samples
Substrate solution is then added and color develops in proportion to the amount of Human CCT8L1
Human SFRP1 (Secreted Frizzled Related Protein 1) ELISA Kit Packaging:96 wells PNMT present in the sampleThe Human (SFRP1) Secreted Frizzled Related Protein 1 ELISA Kit measures Secreted Frizzled Related Protein 1 in samples. The plate has been pre coated with Human SFRP1 antibody. SFRP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SFRP1 Antibody is added and binds to SFRP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SFRP1 antibody. After incubation unbound
