Equine IL-10 (Interleukin 10) ELISA Kit Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Mouse LEPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYR61

Then Streptavidin-HRP is added and binds to the Biotinylated IL-15 antibody

And then biotinylated Human IL1B Antibody is added and binds to IL1B in the sample

The plate has been pre-coated with Human GJA4 antibody

Equine IL-10 (Interleukin 10) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Equine IL 10 (Interleukin 10) ELISA Kit measures IL 10 in Equine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection