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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF5
only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color
Then a biotinylated detection antibody specific for Human VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Rat LGALS3BP in the samples is then determined by comparing the OD of the samples to the standard curve
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CHI3L1
XBP1 (X-Box Binding Protein 1) ELISA Kit (Small Sample) TIMP Standards or samples are addedThe XBP1 (X Box Binding Protein 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human XBP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human XBP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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