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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1 Beta
Then Streptavidin-HRP is added and binds to the Biotinylated FRA-1 antibody
CATHL1 present in the sample is added and binds to antibodies coated on the wells
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DEFa5
Then a biotinylated detection antibody specific for Rat GPX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat CUBN (Cubilin) ELISA Kit Packaging:96 wells The micro ELISA plate providedThe Rat (CUBN) Cubilin ELISA Kit measures Cubilin in Rat samples. The plate has been pre coated with Rat CUBN antibody. CUBN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CUBN Antibody is added and binds to CUBN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CUBN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
