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The assay plate has been pre-coated with mouse anti-Human E-Cadherin monoclonal antibody
Then a biotinylated detection antibody specific for Rat IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Human TRAIL/TNFSF10
The Human MIF (Macrophage Migration Inhibitory Factor) ELISA kit uses the Sandwich-ELISA principle
The concentration of Mouse MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve
Rat CHGB (Chromogranin B) ELISA Kit (Small Sample) IL-1RT-2 The assay plate has beenThe Rat CHGB (Chromogranin B) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat CHGB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat CHGB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
