Rat IL-1Beta (Interleukin 1 Beta) ELISA Kit EasyStep then added a HRP-conjugated antibody

then added a HRP-conjugated antibody specific to Human E2 to each microplate well and incubated

The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm

You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the OD of the samples to the standard curve

only those wells that contain Human GH and HRP-conjugated antibody will exhibit a change in color

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ANXA1

Rat IL-1Beta (Interleukin 1 Beta) ELISA Kit EasyStep then added a HRP-conjugated antibodyThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat IL 1Beta. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat IL 1Beta. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is