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PROZ present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIGLEC2
Then Streptavidin-HRP is added and binds to the Biotinylated MC3R antibody
And then biotinylated Human GPNMB Antibody is added and binds to GPNMB in the sample
Substrate solution is then added and color develops in proportion to the amount of Human SCHIP1
Rat ADCYAP1 (Pituitary adenylate cyclase-activating polypeptide) ELISA Kit Packaging:96 wells PROZ present in the sampleThe Rat ADCYAP1 (Pituitary adenylate cyclase activating polypeptide) ELISA Kit measures ADCYAP1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Adcyap1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
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