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The assay plate has been pre-coated with mouse anti-Human CD55 monoclonal antibody
Then a biotinylated detection antibody specific for Mouse PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
and the color intensity is directly proportional to the amount of Mouse TNFR-2 present
The concentration of Human FCN2 in the samples is then determined by comparing the OD of the samples to the standard curve
COL4 (Collagen Type IV) ELISA Kit (Small Sample) Hepapoietin You can carry dozens inThe COL4 (Collagen Type IV) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
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