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Substrate solution is then added and color develops in proportion to the amount of Human APBA2
The OD value is proportional to the concentration of Human NTXI
You can calculate the concentration of Porcine IL-1Alpha in the samples by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Bovine MB antibody
The concentration of Mouse sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve
Human CNN1 (Calponin 1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (CNN1) Calponin 1 ELISA Kit measures Calponin 1 in samples. The plate has been pre coated with Human CNN1 antibody. CNN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CNN1 Antibody is added and binds to CNN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CNN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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