Rat DAF (Decay Accelerating Factor) ELISA Kit MBPD The concentration of Human MEFV

The concentration of Human MEFV in the samples is then determined by comparing the OD of the samples to the standard curve

Substrate solution is then added and color develops in proportion to the amount of Human MC3R

Then Streptavidin-HRP is added and binds to the Biotinylated ADRA2A antibody

Then Streptavidin-HRP is added and binds to the Biotinylated MGAT4C antibody

The OD value is proportional to the concentration of Human TUBAlpha3C

Rat DAF (Decay Accelerating Factor) ELISA Kit MBPD The concentration of Human MEFVThe Rat DAF (Decay Accelerating Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat DAF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DAF and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate