Human OCLN (Occludin) ELISA Kit KSP3 Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human ERAP1

Calculate the concentration of HDAC2 in the sample by plotting standard curve

then it binds with OVM-IgA bound to precoated antibody

it binds with ATF1 bound to precoated antibody

And then biotinylated Mouse SELE Antibody is added and binds to SELE in the sample

Human OCLN (Occludin) ELISA Kit KSP3 Substrate solution is then addedThe Human OCLN (Occludin) ELISA Kit measures OCLN in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OCLN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.