Human HSPA1B (Heat Shock 70kDa Protein 1B) ELISA Kit HL-1 AFF2 present in the sample

AFF2 present in the sample is added and binds to antibodies coated on the wells

only those wells that contain Mouse LL-37

Substrate solution is then added and color develops in proportion to the amount of Human ARC

Universal HIS in the sample or standard competes with a fixed amount of Universal HIS on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal HIS

Then a biotinylated detection antibody specific for Rat TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human HSPA1B (Heat Shock 70kDa Protein 1B) ELISA Kit HL-1 AFF2 present in the sampleThe Human (HSPA1B) Heat Shock 70kDa Protein 1B ELISA Kit measures Heat Shock 70Kda Protein 1B in samples. The plate has been pre coated with Human HSPA1B antibody. HSPA1B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HSPA1B Antibody is added and binds to HSPA1B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HSPA1B antibody. After incubation unbound Streptavidin HRP